Molecular and Phytochemical Investigation of Angelica dahurica and Angelica pubescentis Essential Oils and Their Biological Activity against Aedes aegypti, Stephanitis pyrioides, and Colletotrichum Species

Abstract

In this study, Angelica dahurica and Angelica pubescentis root essential oils were investigated as pest management perspectives, and root samples were also analyzed genetically using the nuclear ribosomal internal transcribed spacer (ITS) region as a DNA barcode marker. A. pubescentis root essential oil demonstrated weak antifungal activity against Colletotrichum acutatum, Colletotrichum fragariae, and Colletotrichum gloeosporioides, whereas A. dahurica root essential oil did not show antifungal activity. Conversely, A. dahurica root essential oil demonstrated better biting deterrent and insecticidal activity against yellow fever mosquito, Aedes aegypti, and azalea lace bugs, Stephanitis pyrioides, than A. pubescentis root oil. The major compounds in the A. dahurica oil were found as -pinene (46.3%), sabinene (9.3%), myrcene (5.5%), 1-dodecanol (5.2%), and terpinen-4-ol (4.9%). -Pinene (37.6%), p-cymene (11.6%), limonene (8.7%), and cryptone (6.7%) were the major compounds found in the A. pubescentis oil. In mosquito bioassays, 1-dodecanol and 1-tridecanol showed antibiting deterrent activity similar to the positive control DEET (N,N-diethyl-3-methylbenzamide) at 25 nmol/cm2 against Ae. aegypti, whereas only 1-tridecanol showed repellent activity in human-based cloth patch bioassay with minimum effective dosages (MED) of 0.086 0.089 mg/cm2 (DEET = 0.007 0.003 mg/cm2). In larval bioassays, 1-tridecanol was more toxic with an LC50 value of 2.1 ppm than 1-dodecanol having an LC50 value of 5.2 ppm against 1-day-old Ae. aegypti larvae. 1-Dodecanol and 1-tridecanol could be useful for the natural mosquito control agents.

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Document Details

Document Type
Technical Report
Publication Date
Aug 18, 2014
Accession Number
ADA622154

Entities

People

  • Abbas Ali
  • Betül Demirci
  • Blair J. Sampson
  • Chris Werle
  • David E. Wedge
  • Ikhlas A. Khan
  • Natascha Techen
  • Nurhayat Tabanca
  • Ulrich R. Bernier
  • Zengping Gao

Organizations

  • Agricultural Research Service

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Alkanes
  • Alkenes
  • Antifungal Agents
  • Biological Products
  • Chemical Synthesis
  • Chemistry
  • Fatty Acids
  • Fungi
  • Health Services
  • Hydrocarbons
  • Organic Chemistry
  • Pest Control
  • Pharmacies
  • United States

Fields of Study

  • Agricultural and Food sciences

Readers

  • Vector-Borne Disease and Entomology

Technology Areas

  • Biotechnology