Construction of a Cell Based Sensor for the Detection of Autoinducer-2 (Reprint)

Abstract

Microbially Induced Corrosion (MIC) occurs at metal surfaces and is associated with microorganisms and their metabolic activities. These microbes can coexist as biofilms, growing as synergistic communities (consortia) that are able to affect electrochemical processes, both cathodic and anodic, often through co-operative metabolism. Recent research has revealed the role of "quorum sensing" molecules in control of microbial activities such as biofilm formation. In this paper, we propose the detection of quorum sensing molecules as a means of detecting bacterial contamination prior to the onset on biofilm formation. Further we outline the development of an E. coli cell based sensor for detection of the quorum sensing molecule Autoinducer-2 (AI-2).

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2012
Accession Number
ADA622352

Entities

People

  • Chen-yu Tsao
  • Christian J. Sund
  • Christopher M. Byrd
  • Matthew D. Servinsky
  • Patrick C. Allen
  • William E. Bentley

Organizations

  • United States Army Research Laboratory

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Bacteria
  • Construction
  • Contamination
  • Corrosion
  • Detection
  • Detectors
  • Fuel Systems
  • Fuels
  • Material Degradation Processes
  • Microorganisms
  • Military Research
  • Molecules
  • Petroleum
  • Petroleum Industry
  • Proteins
  • Universities

Fields of Study

  • Biology

Readers

  • Gulf War Illness and Chronic Multisymptom Illness in Veterans.
  • Materials Science and Engineering.
  • Military/Explosive Ordnance Disposal (EOD) Technology

Technology Areas

  • Biotechnology