Development of Nanomedicines for Treatment of Posttraumatic Osteoarthritis
Abstract
During this funding period, N-(2-hydroxypropyl)methacrylamide (HPMA) copolymers dexamethasone conjugates were synthesized and labeled with near-infrared dye IRDye CW 800 or Alexa Fluor 647 (P-Dex-IRDye or P-Dex-Alexa). Mice with surgical destabilization of the medial meniscus (DMM) were established as an osteoarthritis model for our evaluation. P-Dex-IRDye and P-Dex- Alexa were given to the DMM mice via intraarticular injection. Optical imaging data suggest that the DMM joints were able to retain the injected P-Dex-IRDye. The treated joints have been isolated for immunohistochemistry and flow cytometry analysis to identify cell phenotypes that internalize the P-Dex-Alexa.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2015
- Accession Number
- ADA622384
Entities
People
- Dong Wang
- P. E. Purdue
- Steven R. Goldring
Organizations
- University of Nebraska Medical Center