Development of Advanced Technologies for Complete Genomic and Proteomic Characterization of Quantized Human Tumor Cells
Abstract
This research proposal provides important insights into cancer mechanisms and blood biomarkers to assess progression and stratification of human glioblastoma. We have successfully established several patient-derived cell lines from glioblastoma tumors and further established a number of quantized cell populations from these parental tumor cell lines. The cell populations have been used for extensive molecular characterization, including whole genome sequencing, transcriptomic analysis, proteomics (secretome as well as N-glycocapture analysis), and high-throughput drug screening for evaluating the effectiveness of drug candidates in targeting these cell populations. Combined, this program significantly advances genomic, proteomic and single-cell technologies. Although the focus of this research is glioblastoma, the proposed tools are generally applicable to all cancer-based studies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2015
- Accession Number
- ADA622404
Entities
People
- Charles Cobbs