Forisome Based Smart Materials

Abstract

The project aimed to unravel the molecular mechanism of structure alteration and force generation of protein actuators called forisomes. The proteins undergo a rapid calcium induced ATP independent anisotropic contraction. We aimed to express recombinant forisome proteins in a cell free wheat germ system and crystallize the proteins for x-ray diffraction and electron microscopy. While expression of the proteins was achieved, the conditions in the wheat germ system prevented crystallization. Therefore we expressed proteins carrying His-tags to purify proteins. Purified proteins are currently subjected to various conditions for crystallization.

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Document Details

Document Type
Technical Report
Publication Date
Mar 31, 2015
Accession Number
ADA623387

Entities

People

  • Hanjo Hellmann
  • Michael Knoblauch

Organizations

  • Washington State University

Tags

Communities of Interest

  • Human Systems

DTIC Thesaurus Topics

  • Abstracts
  • Agreements
  • Amino Acids
  • Biological Sciences
  • Crystallization
  • Crystals
  • Department Of Defense
  • Electron Microscopy
  • Engineering
  • Materials
  • Mathematics
  • Production
  • Production Engineering
  • Proteins
  • Students
  • X Rays
  • X-Ray Diffraction

Readers

  • Molecular and Cellular Biochemistry

Technology Areas

  • Microelectronics