Laboratory Validation of the Sand Fly Fever Virus Antigen Assay

Abstract

Sandfly fever group viruses in the genus Phlebovirus (family Bunyaviridae) are widely distributed across the globe and are a cause of disease in military troops and indigenous peoples. We assessed the laboratory sensitivity and specificity of the Sand Fly Fever Virus Antigen Assay, a rapid dipstick assay designed to detect sandfly fever Naples virus (SFNV) and Toscana virus (TOSV) against a panel of phleboviruses. The assay detected SFNV and TOSV, as well as other phleboviruses including Aguacate, Anahanga, Arumowot, Chagres, and Punta Toro viruses. It did not detect sandfly fever Sicilian, Heartland, Rio Grande, or Rift Valley fever viruses. It did not produce false positive results in the presence of uninfected sand flies (Lutzomyia longipalpis) or Cache Valley virus, a distantly related bunyavirus. Results from this laboratory evaluation suggest that this assay may be used as a rapid field-deployable assay to detect sand flies infected with TOSV and SFNV, as well as an assortment of other phleboviruses.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2015
Accession Number
ADA626599

Entities

People

  • Kristen L. Burkhalter
  • Mitchell S. Szymczak
  • Myrna M. Miller
  • Will K. Reeves

Organizations

  • United States Air Force School of Aerospace Medicine

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Antigens
  • Arboviruses
  • Bunyaviridae
  • Bunyaviridae Infections
  • Central Nervous System
  • Culture Techniques
  • Diseases And Disorders
  • Phlebotomus Fever
  • Public Health
  • Rift Valley Fever
  • Rift Valleys
  • Sensitivity
  • Test And Evaluation
  • Validation
  • Valleys
  • Virus Diseases
  • Viruses

Fields of Study

  • Biology

Readers

  • Vector-Borne Disease and Entomology
  • Virology (or Medical Virology).