Applications and Methods for Continuous Monitoring of Physiological Chemistry

Abstract

The overall goal of this project was to develop a platform methodology to clone, evolve, purify and stabilize high activity oxidase enzymes that are suitable for point-of-care diagnostic and human biosensor applications. The general approach was to add fusion proteins to increase enzyme stability without compromising enzyme activity. Oxidase enzymes that recognize lactate and histamine were improved, and an effort was made to develop a cortisol oxidase from cholesterol oxidase. These studies set the stage for a platform methodology to create biorecognition elements that are suitable for a wide range of point-of-care diagnostic devices.

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Document Details

Document Type
Technical Report
Publication Date
Feb 04, 2016
Accession Number
ADA627115

Entities

People

  • Daniel Aillon
  • David A. Johnson
  • Mark Richter
  • Peter A. Petillo
  • Philip Gao

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Alcohols
  • Amino Acids
  • Biomedical And Dental Materials
  • Biosensors
  • Chemical Synthesis
  • Chemistry
  • Crystal Structure
  • Detection
  • Detectors
  • Engineering
  • Health Services
  • Manufacturing
  • Molecular Biology
  • Monitoring
  • Organic Chemistry
  • Point-Of-Care Diagnostic Testing
  • Proteins

Fields of Study

  • Engineering

Readers

  • Distributed Systems and Data Platform Development
  • Molecular and Cellular Biochemistry

Technology Areas

  • Biotechnology