Acute Respiratory Distress Syndrome Secondary to Inhalation of Chlorine Gas in Sheep

Abstract

Toxic industrial chemicals (TICs) are potential terrorist weapons. Several TICs, such as chlorine, act primarily on the respiratory tract, but knowledge of the pathophysiology and treatment of these injuries is inadequate. This study aims to characterize the acute respiratory distress syndrome (ARDS) caused by chlorine gas (Cl2) inhalation in a large-animal model. Methods: Anesthetized female sheep were ventilated with 300 L of a Cl2 /air/ oxygen mixture for 30 minutes. In phase 1 (n 35), doses were 0 ppm (Group 1, n = 6); 120 ppm (Group 2, n = 6); 240 to 350 ppm (Group 3, n = 11); and 400 to 500 ppm (Group 4, n = 12). In phase 2 (n = 17), doses were 0 ppm (Group 5, n = 5); 60 ppm (Group 6, n = 5); and 90 ppm (Group 7, n = 7), and the multiple inert gas elimination technique (MIGET) was used to characterize the etiology of hypoxemia. Computed tomography (CT) scans were performed daily for all animals. Results: In Phase 1, lung function was well maintained in Group 1; Cl2 caused immediate and sustained acute lung injury (PaO2-to-FiO2 ratio, PFR less than 3.0) in Group 2 and ARDS (PFR less than 2.0) in Groups 3 and 4. All animals in Groups 1 and 2 survived 96 hours. Kaplan-Meier analysis showed dose- related differences in survival (log-rank test, p less than 0.0001). Logistic regression identified 280 ppm as the lethal dose 50%. CT and histopathology demonstrated lesions of both small airways and alveoli. In Phase 2, MIGET showed diversion of blood flow from normal to true-shunt lung compartments and, transiently, to poorly ventilated compartments. Conclusions: Cl 2 causes severe, dose-related lung injury, with features seen in both smoke inhalation and in ARDS secondary to systemic disease. This model will be used to test new therapeutic modalities.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2006
Accession Number
ADA628028

Entities

People

  • Andriy I Batchinsky
  • Bryan S. Jordan
  • Candace A. Baird
  • David K. Martini
  • Denise E. Hardin
  • Edward J. Dick
  • James Fudge
  • Leopoldo C. Cancio

Organizations

  • United States Army Institute of Surgical Research

Tags

DTIC Thesaurus Topics

  • Acute Respiratory Distress Syndrome
  • Anesthesia
  • Arteries
  • Blood
  • Cardiovascular Physiological Phenomena
  • Chemistry
  • Detectors
  • Health Services
  • Lung Diseases
  • Medical Personnel
  • Wounds And Injuries
  • X-Ray Computed Tomography

Fields of Study

  • Medicine

Readers

  • Combustion science or combustion engineering.
  • Immunology and Pathology
  • Toxicology/Environmental Toxicology