Lecithin:retinol acyltransferase in ARPE-19

Abstract

The purpose of this study is to investigate if a readily available cell line (APRE 19) may be used to study in vitro function of visual cycle enzymes such as lecithin:retinol acyltransferase (LRAT). Cells incubated with exogenous retinol accumulated intracellular all trans retinol and all trans retinyl ester. Membrane proteins from ARPE 19 exhibited LRAT activity, which was inhibited by an LRAT inhibitor, retinyl bromoacetate (RBA). Gene microarray and Western blot results indicated that ARPE 19 cells expressed LRAT transcript and the LRAT protein. Therefore, our data show that ARPE 19 contains an active LRAT enzyme and suggest that it is an appropriate cell system to study visual cycle enzymes.

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Document Details

Document Type
Technical Report
Publication Date
Apr 05, 2005
Accession Number
ADA628095

Entities

People

  • Andrew Tsin
  • Phillip D. Bowman
  • Simon G. Trevino
  • Steven T. Schuschereba

Organizations

  • United States Army Institute of Surgical Research

Tags

DTIC Thesaurus Topics

  • Albumins
  • Antibodies
  • Biochemistry
  • Biological Pigments
  • Carrier Proteins
  • Cell Line
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Detection
  • Epithelial Cells
  • Epithelium
  • Fatty Acids
  • Membrane Proteins
  • Neuroglia
  • Plant Extracts
  • Proteins

Fields of Study

  • Biology

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