Elimination of Matrix-Based Interferences to a Fluorescent Nitrite/Nitrate Assay by a Simple Filtration Procedure

Abstract

Pathophysiological levels of oxygen radical metabolites have been studied as indicators of trauma caused by burn insult. The 2,3-diaminonaphthalene assay is routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. Several laboratories, including ours, have noted matrix-based interferences resulting in decreased assay sensitivity during nitrite/ nitrate analysis. We evaluated filtration using Millipore Ultrafree-MC 10,000 NMWL filters for the ability to eliminate matrix-based interferences from human serum and tissue culture medium, thereby restoring assay sensitivity.

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Document Details

Document Type
Technical Report
Publication Date
Aug 15, 2000
Accession Number
ADA628392

Entities

People

  • Anthony E. Pusateri
  • David W. Bedwell
  • Gerald A. Merrill
  • Victor R. Rivera

Organizations

  • United States Army Institute of Surgical Research

Tags

DTIC Thesaurus Topics

  • Albumins
  • Blood
  • Body Fluids
  • Capillary Electrophoresis
  • Chemistry
  • Chromatographic Analysis
  • Coefficients
  • Detection
  • Filtration
  • Free Radicals
  • Liquid Chromatography
  • Macromolecules
  • Molecules
  • Proteins
  • Standards
  • Statistical Analysis
  • Tissue Culture

Readers

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