Sequential Delivery of BMP-2 and IGF-1 Using a Chitosan Gel with gelatin Microspheres Enhances Early osteoblastic Differentiation

Abstract

The purpose of this study was to develop and characterize a chitosan gel/gelatin microsphere (MSs) dual delivery system for sequential release of bone morphogenetic protein 2 (BMP 2) and insulin like growth factor 1 (IGF 1) to enhance osteoblast differentiation in vitro. We made and characterized the delivery system based on its degree of cross linking, degradation, and release kinetics. We also evaluated the cyto-toxicity of the delivery system and the effect of growth factors on cell response using pre osteoblast W 20 17 bone marrow stromal cells. IGF 1 was first loaded into Mss, and then the IGF 1 containing MSs were encapsulated into the chitosan gel which contained BMP 2.

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Document Details

Document Type
Technical Report
Publication Date
Jan 18, 2012
Accession Number
ADA630574

Entities

People

  • Chad A. Krueger
  • Di Chen
  • John B Holcomb
  • Joseph C Wenke
  • Milan Sen
  • Sungwoo Kim
  • Yunqing Kang
  • Yunzhi Yang

Organizations

  • United States Army Institute of Surgical Research

Tags

DTIC Thesaurus Topics

  • Acetic Acid
  • Amino Acids
  • Cells
  • Culture Techniques
  • Electron Microscopes
  • Electron Microscopy
  • Growth Factors
  • Materials
  • Microscopes
  • Microscopy
  • Microspheres
  • Peptide Growth Factors
  • Polymers
  • Polysaccharides
  • Proteins
  • Scanning Electron Microscopes
  • Scanning Electron Microscopy

Readers

  • Molecular and Cellular Biology
  • Nanocomposite Materials Science
  • Oncology (Cancer Research).