In Vitro Chromosome Aberrations Study in Chinese Hamster Ovary (CHO) Cells
Abstract
In the Chinese Hamster Ovary (CHO) cell chromosome aberration assay, trifluoromethane (FE-13) induced a significant increase in clastogenic damage at concentrations in air ranging from 80.0% to 100.0% in the absence of metabolic activation. Under these same conditions, 100% nitrogen also induced a significant increase in chromosomal damage to the same magnitude, suggesting the possibility that the response may reflect changes in oxygen levels rather than FE-13 specifically. In the presence of metabolic activation, FE-13 and 100.0% nitrogen induced a non significant increase in chromosomal damage. It should be noted that the level of induced damage was about the same in both the non activated and the S9 activated FE-13 treated cultures while the percentage of aberrant cells was 0.0% and 2.0% in the non activated and S9 activation control cultures, respectively, suggesting that this may account for the statistically positive increase without S9 and a statistically non significant increase with S9.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 24, 1996
- Accession Number
- ADA630794
Entities
People
- Anuradha G. Udumudi
- Leslie Hill
- Paul W. Andrews
- Raymond R. Tice