Hibernation-Based Therapy to Improve Survival of Severe Blood Loss
Abstract
Our first granting quarter was spent getting set-up administratively as well as logistically and starting our rabbit ear vein studies. Our second and third granting quarters were spent completing the rabbit ear vein studies and starting our pig model experiments. Previous studies had showed a local irritation at the site of injection and it was thought to be associated with the hyper-tonicity of the study drug 4M beta-hydroxybutyrate, 43mM melatonin in 20% DMSO (BHB/M), but no follow-up study had been performed. Our main finding from the rabbit ear vein studies was that BHB/M could be given I.V. at the concentrations stated above if the BHB/M was administered at pH 7.4. Our pig experiments ensued soon after the confirmation from the pathologist regarding our rabbit ear vein findings. After the completion of 18 experiments, it was noted that the study animals did not respond to drug administered intra-osseous in the same manner as animals receiving the drug intra-venous. HPLC analysis of the BHB and melatonin concentrations showed that animals receiving BHB/M intra-osseous did not have the same serum concentrations of BHB and melatonin when compared to animals receiving BHB/M intra-venous.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2011
- Accession Number
- ADA630919
Entities
People
- Gregory Beilman
- Javariah Ashgar
- Kristine Mulier
- Patei Iyegha
Organizations
- University of Minnesota