Metal Ion Sensor with Catalytic DNA in a Nanofluidic Intelligent Processor
Abstract
This paper presents the potential of combining Pb2+ specific catalytic DNA enzymes with microfluidic devices. We incorporate the catalytic DNAzyme into a detection channel on the microfluidic device. Lead containing solution is placed in a source channel. By appropriate application of voltages across channels, the lead solution can be injected through nanocapillary array interconnects (NAI) that separate the channels. These gatable microfluidic devices build upon research developed by Bohn and Sweedler et al. (16-18). Methods for adapting lead specific biosensors to the NAI microfluidic device are presented. Performances of the developed method were evaluated by studying quantifiable concentration range, method accuracy and precision. Successful application to real sample analysis using an electroplating sludge certified reference material is also presented.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 08, 2005
- Accession Number
- ADA631384
Entities
People
- Carla Swearingen
- Daryl Wernette
- Donald M. Cropek
- Jonathan V. Sweedler
- Paul W. Bohn :in-hyoung
- Yi Lu
Organizations
- Construction Engineering Research Laboratory