Profiling of Urine Using ProteinChip Technology

Abstract

Urine is an extremely valuable sample type for biomarker discovery due to the non-invasive collection and the relatively low protein content, which makes detection of perturbations associated with disease easier. SELDI-TOF analysis is ideally suited for analysis of urine since the chromatographic capture mechanism can tolerate salt and urea in the urine sample that would otherwise need to be removed prior to mass spectrometric analysis. While neat urine can be analyzed directly on ProteinChip arrays, urine can also benefit from an enrichment step, which has been shown to increase the number of proteins detected more than twofold. Because urine volume and contents can vary substantially between individuals and within individuals over time, sample collection and storage should be carefully controlled to assure reproducible and clinically relevant results.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2012
Accession Number
ADA633090

Entities

People

  • Amanda L. Bulman
  • Diane L. Mccarthy
  • Ronald L. Woodbury

Organizations

  • United States Army Institute of Surgical Research

Tags

DTIC Thesaurus Topics

  • Acetonitrile
  • Albumins
  • Alcohols
  • Centrifuges
  • Chemical Synthesis
  • Chemistry
  • Citric Acid
  • Ethylene Glycol
  • Ion Exchange
  • Mass Spectrometry
  • Materials
  • Molecular Biology
  • Nitriles
  • Organic Chemistry
  • Organic Solvents
  • Proteins

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