Etiology and Effects of Ciomiphene on Cystic Endometrial Hyperplasia in the Miniature Pig

Abstract

Cystic endometrial hyperplasia (CEH) of unknown etiology has been discovered in Swine Leukocyte Antigen (SLA) inbred miniature pigs. In humans endometrial hyperplasia is known most often to be associated with hyperestrogenism and thus it was hypothesized that CEH in SLA miniature pigs may also be due to hyperestrogenism. The present investigation was undertaken to perform a histological and biochemical characterization of CEH in miniature pigs. The primary objectives of this study were to: 1) document the gross morphological and histological changes of the endometrium associated with CEH, 2) determine whether differences exist in hormonal and/ or steroid hormone receptor concentrations between CEH and non-CEH animals, 3) determine whether CEH can be induced by unopposed estrogen action and 4) examine the effect(s) of progesterone and clomiphene citrate on the progression of existing CEH condition. The SLA miniature sows were assigned as CEH or non-CEH, based on the examination of the uterus by a midventral laparotomy. When necessary, animals were bilaterally ovariectomized during the laparotomy. Serum concentrations of estrone (El), estradiol (E2), progester one (P), testosterone (T) and luteinizing hormone (LH) were measured by specific radioimmunoassays (RIA). Uterine tissue samples for histology and for quantitation of receptors were collected from sows at necropsy. Sodium dodecyl sulfate . (SDS)-polyacrylamide gel electrophoresis was used for the qualitative analysis of proteins in cyst fluid, uterine flushings and serum samples. Endometrial estrogen and progesterone receptors were quantitated by radiolabelled ligand binding assay. Hematoxylin and eosin staining of tissue was utilized for the histological analysis.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Dec 17, 1986
Accession Number
ADA633986

Entities

People

  • Chhanda Dutta

Organizations

  • Uniformed Services University of the Health Sciences

Tags

DTIC Thesaurus Topics

  • Albumins
  • Animal Structures
  • Blood
  • Body Weight
  • Cardiovascular Diseases
  • Cardiovascular Physiological Phenomena
  • Cardiovascular System
  • Cells
  • Chemistry
  • Endocrine Glands
  • Estrogens
  • Health Services
  • Progesterone
  • Regression Analysis
  • Rodents
  • Sex Glands
  • Urogenital System

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Trauma Surgery or Emergency Medicine.
  • Women's Health and Cancer Risk Research: African American Women and Pregnancy Outcomes.