Differential Expression of Inward and Outward Potassium Currents in the Macrophage-like Cell Line J774.1

Abstract

The electrophysiological properties of the J774.1 macrophage-like cell line were studied at 25 deg C. Both conventional intracellular microelectrode and whole-cell patch microelectrode techniques were used. Long-term adherent (>24 hours) cultures investigated with intracellular electrodes exhibited a broad distribution of resting membrane potentials and membrane resistances (RMP approximately -50 +/- 3.0 mV, Rm approximately 117 +/- 13 Mohm, Mean +/- SEM, n approximately 55). Most cells with hyperpolarized RMPs exhibited inward rectification.

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Document Details

Document Type
Technical Report
Publication Date
Apr 02, 1985
Accession Number
ADA634068

Entities

People

  • Paul A. Sheehy

Organizations

  • Uniformed Services University of the Health Sciences

Tags

DTIC Thesaurus Topics

  • Blood
  • Cardiovascular System
  • Cell Line
  • Cell Membrane
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Culture Techniques
  • Health Services
  • Lymphocytes
  • Macrophages
  • Membrane Potentials
  • Muscle Fibers
  • Phagocytes
  • Rodents

Fields of Study

  • Biology

Readers

  • Electrochemical Surface Science
  • Immunology
  • Neuroscience