Nucleotide Sequence of the Hantaan Virus S RNA Segment and Expression of Encoded Proteins
Abstract
The determination of the nucleotide sequence of the Hantaan virus (HTV) genome M RNA segment was begun to devise a strategy for expressing the encoded proteins in a eukaryotic expression system and to allow a comparison with other HTV RNA segments. Concurrently, the complete nucleotide sequence of the HTV S RNA segment was determined from three overlapping eDNA clones. The S RNA segment was 1695 nucleotides in length encoding two possible proteins in a unique coding strategy for members of the Bunyaviridae family. A large open reading frame (ORF) followed by a second smaller ORF was found in the viral complementary-sense RNA. Complementary sequences were found at the 3' and 5' termini of the RNA molecules which would allow formation of a stable stem-loop structure and the formation of the circular nucleocapsid. The M and S RNA segments contained 3' noncoding regions of similar length with 20 of the nucleotides conserved. Conserved sequences which could act as possible transcription termination signals in the 5' noncoding regions were also observed.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 03, 1987
- Accession Number
- ADA634961
Entities
People
- Gerald B. Jennings
Organizations
- Uniformed Services University of the Health Sciences