The Intracellular Free Amino Acid Pool Represents Tracer Precursor Enrichment for Calculation of Protein Synthesis in Cultured Fibroblasts and Myocytes
Abstract
In summary, we validated intracellular free amino acid enrichment as surrogate for precursor enrichment for protein synthesis in cell culture. In both fibroblasts and myocytes, protein synthesis rates calculated from intracellular free amino acid enrichments were close to the true values of protein synthesis, irrespective of the tracer. Using extracellular amino acid enrichments resulted in underestimation of protein synthesis. This approach is applicable to in vivo studies in human subjects.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2004
- Accession Number
- ADA635656
Entities
People
- David L. Chinkes
- Robert R Wolfe
- Wenjun Zhou Martini
Organizations
- United States Army Institute of Surgical Research