NF-kB Activation as a Biomarker of Light Injury Using a Transgenic Mouse Model

Abstract

The spatial and temporal activation of NF-kB (p65) was monitored in the retina of a transgenic mouse model (cis-NF-kB-EGFP) in vivo after receiving varying grades of laser induced thermal injury in one eye. Baseline retinal images were collected from 26 mice and were reimaged up to five months later after receiving thermal injury using a Heidelberg Spectralis HRA confocal scanning laser ophthalmoscope (cSLO) with a spectral domain optical coherence tomographer (SD-OCT). Injured and control eyes were enucleated at discrete time points following laser exposure for cryosectioning to determine localization of NF-kB dependent enhanced green fluorescent protein (EGFP) reporter gene expression in the retina using fluorescence microscopy.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2012
Accession Number
ADA637337

Entities

People

  • Adam Boretsky
  • Dallas Golden
  • Ginger M. Pocock
  • Gracie Vargas
  • Heuy-ching Wang
  • Jeffrey W. Oliver
  • Massoud Motemedi
  • Praveena Gupta

Organizations

  • United States Army Institute of Surgical Research

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DTIC Thesaurus Topics

  • Abstracts
  • Availability
  • Biological Markers
  • Classification
  • Contracts
  • Fluorescence
  • Gene Expression
  • Information Operations
  • Instructions
  • Laser Safety
  • Microscopy
  • Monitoring
  • Scanning
  • Security
  • United States

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  • Breast cancer cell signaling and growth regulation.
  • Medical Imaging.
  • Vision Science/Vision Psychology/Cognitive Neuroscience.

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  • Directed Energy