Detection and Measurement of Antibody-Antigen Reactions.
Abstract
The current investigation involves an application of a technique developed by Walter B. Dandliker, Ph. D., Professor of Biochemistry, University of Miami School of Medicine. This involves measurements of fluorescence polarization and/or intensity measurements made on solutions which contain fluorescent-labelled antigens or antibodies. This technique was studied in great detail by Doctor Dandliker using fluorescein-labelled ovalbumin and rabbit antifluorescein ovalbumin. Fluorescence polarization measurements were made with a modified light-scattering apparatus. His need for more sophisticated and accurate optical equipment soon became obvious and an improved polarometer was built for by Cordis Corporation. Keywords: U/A Reports, Antibody-antigen reactions, Biochemistry, Chemical analysis, Humoral immunity cellular, Immunity.(jg)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 08, 1962
- Accession Number
- ADA955928
Entities
People
- William P. Murphy Jr.