Search for Hepatitis A Viruses by New Methods
Abstract
Recent studies have shown that patients who have recovered from HBV infections develop a specific cellular cytotoxicity toward the PLC/PRF/5 hepatoma cell line. These observations were extended by analysis of possible specific cellular cytotoxicity in three persons passively immunized with anti- HBs globulin and in 20 individuals vaccinated with HBsAg vaccine. The three subjects with passively administered antibody activity were negative for cellular activity, but in 2 of 20 individuals vaccinated with formalinized HBsAg vaccine, a specific cytotoxicity for hepatoma target cells was noted. To facilitate testing of large numbers of mouse antisera, a micro solid-phase radioimmunometric assay (micro-SPRIA) and an enzyme-linked immunosorbent assay (ELISA) were developed and compared to the commercial radioimmunoassay (Ausab) for detection of anti-HBs antibody. The two solid-phase antibody assays proved to be 200-350X more sensitive for detection of antibody than the commercial antibody assay kit. Preliminary data were presented in conjunction with an undenatured P22-P25 polypeptide vaccine prepared by the technology of Zuckerman and coworkers. The polypeptide material, isolated in micelle form, retained high levels of HBsAg activity when tested by solid-phase radioimmunoassay in contrast to the low activity observed with SDS-denatured material. This material has been inoculated into mice to test its relative level of immunogenicity.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1981
- Accession Number
- ADB081939
Entities
People
- Joseph L. Melnick
Organizations
- Baylor College of Medicine