Immunity in Dengue
Abstract
Prototype dengue (DEN) 4 (H-241) was received as original viremic human serum and passed once in a susceptible monkey and twice in Aedes albopictus replicated in primary dog kidney and African green monkey kidney cells and characterized at passages 7, 15, 30 and 50. Serial weekly passage of undiluted virus was carried to the 50th passage in both primary cell cultures. Parental DEN-4 phenotype included large plaque formation in LLC-QMK2 cells, plaque formation in GMK cells, cytopathic effect in LLC-MK2, growth in human monocyte cultures, growth at 39 C, consistent production of viremia in monkeys and short-incubation neurovirulence in mice. By 7 passages in both cell cultures, DEN-4 viruses exhibited reduced plaque-size in LLC-MK2, failure to plaque in GMK, to produce CPE in LLC-MK2, or to grow in human monocytes. Serial passage in PDK as opposed to GMK, resulted in a graduated loss of monkey virulence. PDK 50 virus did not replicate at 39 C and only 1 of 4 inoculated monkeys developed an antibody response.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1983
- Accession Number
- ADB097240
Entities
People
- Scott B. Halstead
Organizations
- University of Hawaiʻi System