Production of a Suitable Antibody and an Enzyme Immunoassay Kit for the Field Detection of T-2 Toxin
Abstract
Phospholipid vesicles, liposomes, were used as an immune enhancement (adjuvant) medium to produce antibodies specific for T-2 toxin. Vesicles were prepared such that neat T-2 toxin and a solution of T-2 succinyl bovine serum albumin were each either encapsulated or adsorbed on vesicles. Permutations of these formulations were used to immunize mice by repeated subcutaneous injections. An enzyme-linked immunosorbent assay was developed and used to assay serum from immunized mice for T-2 specific antibody. The assay allows for direct measurement of T-2 antibody, with the capability for competitive inhibition studies using T-2 standard solutions. Two objectives were specified for this study. They were to produce antibodies specific for T-2 toxin, and to demonstrate an immune enhancement, with respect ot T-2 antibody production, from the vesicle-based antigen delivery method. The vesicle delivered antigen does produce T-2 specific antibodies to T-2 toxin. Potential benefit of using the vesical base immunization method of increasing the rate of antibody production or increasing antibody titers is still under study. Keywords: Vesicle microencapsulation; Enzyme-Linked Immunosorbent Assay (ELISA); and Field detection.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 25, 1983
- Accession Number
- ADB101431
Entities
People
- Leroy L. Richer
- Richard A. Callahan