Cloning of Acetylcholinesterase Gene in a Microbial Vector.
Abstract
Efforts made toward the end of cloning synthetic deoxyribonucleic acid (DNA) sequences coding for the enzyme acetylcholinesterase are described. The methods described include the isolation, purification, and analysis of messenger ribonucleic acid (mRNA) from the electric organ of Torpedo californica, a tissue which is highly enriched in acetylchloinesterase. Also described is the construction of three libraries of clones of E. coli bacteria containing the synthetic DNA sequences, and the screening that has been done to isolate a clone or clones containing sequences coding for the enzyme. The conclusion is made that the correct clone(s) have not yet been isolated, although the evidence strongly indicates that at least one bank should contain the clone(s) of interest.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 14, 1983
- Accession Number
- ADB107463
Entities
People
- Brian L. West
- John D. Baxter
Organizations
- University of California, San Francisco