Development of Analytical Methods for the Determination of Drug Substances in Biological Fluids.
Abstract
Methods available to quantitate halofantrine, an antimalarial drug with demonstrated activity against Plasmodium falciparum, in blood and plasma are limited. We report the development of a simple and rapid HPLC assay for the analysis of 0.5 ml blood and plasma specimens. The method detection limit is 1 ng/ml for both halofantrine (WR 171,669) and its putative metabolite (WR 178,460). The method's principal techniques include sample cleanup by protein precipitation and by a column elution step, precautions against loss of drug because of absorption to glass, and fluorescence detection. The method proved adequate in tests of linearity, recovery, precision, and accuracy. Drug stability tests of specimens stored at -20C and 80C indicated the necessity of storage at 80C. Routine analysis of human and animal blood plasma samples were satisfactory performed. Keywords: Halofantrine (WR 171,669), Putative Metabolite (WR 178,460); Blood; Blood Plasma.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 13, 1985
- Accession Number
- ADB113975
Entities
People
- Emil T. Lin
- Leslie Z. Benet
- Robert A. Upton
- Winnie L. Gee
Organizations
- University of California, San Francisco