Active Antitoxic Immunization against Ricin Using Synthetic Peptides
Abstract
This year, peptides with overlapping sequences of candidate A-chain peptides, (i.e., 17-31, 84-100, 102-118, 175-191, and 227-243) were synthesized and conjugated to BSA. Immunization studies have been performed on most of the A- and B-chain peptides synthesized for the project. Antisera from the peptides were tested by ELISA for their binding to corresponding immobilized peptides corresponding ricin subunit, and intact ricin. Challenge studies were performed on peptides eliciting cross-reacting antibodies. Although some peptides elicited high-titer, cross-reacting antibodies, none of them were able to provide protection against ricin challenge. Keywords: Ricinus communis; Plant toxins; Ricin, Synthetic peptides, Immunization, RA 1; Antitoxins; Active sites; Binding sites cytoplasm.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1989
- Accession Number
- ADB137490
Entities
People
- Amrit K. Judd
Organizations
- SRI International