Chemical Blistering: Cellular and Macromolecular Components

Abstract

The overall purpose of this investigation is to elucidate the molecular mechanisms by which bis(beta-chloroethyl) sulfide (BCES) exerts its vesicant action when applied topically to human skin. The technical objectives of the project are to establish morphological, cytochemical, and/or biochemical indicators of epidermal mustard toxicity and to examine and evaluate the relevance of these indicators to vesication. The following three biological systems have been used in this study: (a) stratified, cornified primary cultures of keratinocytes grown on nylon microporous membranes positioned at the air- medium interface; (b) submerged monolayer cultures of primary keratinocytes that contain early differentiated as well as germinating cells and are grown in a medium having a low level (0.08-0.10 micronmo) of Ca2(+)(Calcium ions); and (c) cultures of the BSC 40 line of monkey kidney cells grown as a submerged monolayer. Keywords: Mustard, Keratinocyte, Tissue culture, Alkylation, Toxicity, Chemical blistering, Metabolism, DNA Repair, Mitochondria, Epidermal differentiation, RAV, BCES, BSC.

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Document Details

Document Type
Technical Report
Publication Date
Dec 31, 1989
Accession Number
ADB145621

Entities

People

  • I. A. Bernstein

Organizations

  • University of Michigan

Tags

DTIC Thesaurus Topics

  • Alcohols
  • Alkylating Agents
  • Biomedical Research
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Chlorides
  • Deoxyribonucleic Acids
  • Epithelial Cells
  • Growth Factors
  • Health Services
  • Laboratory Animals
  • Mustard Agents
  • Organic Chemistry
  • Synthetic Membranes

Readers

  • Analytical Chemistry
  • Cellular and Molecular Pathways of Apoptosis.
  • Organizational Process Management (OPM).

Technology Areas

  • Biotechnology