Chemical Blistering: Cellular and Macromolecular Components.
Abstract
The overall purpose of this investigation is to elucidate the molecular mechanisms by which bis(beta-chloroethyl) sulfide (BCES) exerts its vesicant action when applied topically to human skin by establishing morphological, cytochemical and/or biochemical indicators of mustard toxicity in cultures of keratinocytes and examining and evaluating the relevance of these parameters to vesication. During the second year of this project, the investigation has attempted to (a) confirm that DNA is the macromolecular system which is most sensitive to damage in cultures exposed to BCES, (b) identify the lowest level of exposure at which the lesion in DNA can be observed and the level of exposure at which the lesion in DNA can be observed and the level of exposure at which the lesion cannot be repaired. Keywords: Mustard, Keratinocyte, Tissue culture, ALkylation, Toxicity, Chemical blistering, Mitochondria, Metabolism, DNA Repair, Epidermal proliferation, Epidermal differentiation. (JES)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 31, 1988
- Accession Number
- ADB145691
Entities
People
- I. A. Bernstein
Organizations
- University of Michigan