Chemical Blistering: Cellular and Macromolecular Components.
Abstract
The overall purpose of this investigation was to elucidate the molecular mechanisms by which bis(beta-chlorethyl)sulfide (BCES) exerts its vesicant action when applied topically to human skin. This was to be done by establishing morphological, cytochemical, and/or biochemical indicators of mustard toxicity in primary monolayer and stratified, cornified cultures of cutaneous keratinocytes and evaluating the relevance of these parameters to vesication. Experiments have shown that DNA metabolism and structure are the initial targets of BCES in both types of culture. Synthesis of DNA is inhibited and the structure of the nucleic acid is damaged by a lower level of BCES than is necessary to inhibit the synthesis of RNA or protein. Keywords: Mustard, Keratinocyte, Tissue culture, Alkylation, Toxicity, Chemical blistering, Mitochondria, Metabolism, DNA Repair, Epidermal proliferation, Epidermal differentiation. (JES)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 31, 1989
- Accession Number
- ADB145992
Entities
People
- I. A. Bernstein
Organizations
- University of Michigan