Determination of the In Vitro and In Vivo Activity of Compounds Tested against Punta Toro Virus
Abstract
Military Relevance: The viruses of military significance targeted by this research are sandfly fever virus and Rift Valley fever virus, both endemic to the Middle Eastern area and capable of severely hampering military operations if an outbreak occurs in susceptible populations. The Punta Toro virus is a closely related virus which is safer to use in the laboratory and which, as target for antiviral agents, has been shown to be highly predictable of efficacy against sandfly and Rift Valley fever viruses. In Vivo Assessment of Lethal Toxicity: Approximately LD50 values were obtained in mice for 105 AVS compounds. In Vitro Evaluation of Test Compounds Against Punta Toro Virus: A total of 342 AVS compounds were evaluated against the Adames strain of PTV using an in vitro assay procedure. This procedure used, as initial endpoint, inhibition of viral- induced cytopathic effect in LLC-MK2 cells, and as a confirming test, reduction in virus yield. 15 compounds were considered to be strongly active against the virus; most were also inhibitory to the Balliet virus strain in confirming experiments. A total of 42 compounds exerted a moderate PTV-inhibitory effect. Effect of AVS Compounds on Hepatotropic Infections in Mice Induced by the Adames Strain of Punta Toro Virus: A total of 882 experiments were run in evaluating 121 AVS compounds against the hepatotropic PTV infection.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 28, 1991
- Accession Number
- ADB157641
Entities
People
- Robert W. Sidwell
Organizations
- Utah State University