Method for Evaluation of Drug Resistance for Plasmodium Falciparum. Phase 2
Abstract
This contract was designed to show the feasibility of a method to more efficiently assess the drug sensitivity of Plasmodium falciparum. The method depends on the differential detection of parasite produced enzyme against a background of host produced enzyme. The basic premise is that any compound with anti-malarial activity should be detected with this enzyme based readout since any anti-malarial activity should lead to lower levels of parasite- produced enzyme in test compared to control cultures. The contract goals were to optimize the conditions for enzyme measurement and then to demonstrate that the enzyme assay could be substituted for existing methods of growth measurement of P. falciparum in vitro. The final goal of this project was to obtain recent isolates of chloroquine-sensitive and chloroquine-resistant strains of P. falciparum and demonstrate that the enzyme measurement system could be used in chloroquine sensitivity assays in vitro.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 15, 1991
- Accession Number
- ADB158380
Entities
People
- Michael T. Makler