Development of a Short-Term In-Vitro Dermal Toxicity Screening Test Utilizing Human Cells. Phase 1.
Abstract
There is a large effort underway in the world today to identify and develop in-vitro alternative tests to supplement or replace current in vivo toxicity test methods. The Agarose Diffusion Method is an excellent candidate for development as a short term in-vitro dermal toxicity screening test using human cells. The Phase I effort has adapted the current Agarose Diffusion Method for use in screening for primary skin irritation. This required manipulation of the agarose thickness and the investigation of an alternative cell type. The human foreskin cell line has been adapted to the test method. Of particular interest is the similarity of the human cell line with the L-929 mouse fibroblast cell line. The test system developed employed an agarose overlay medium volume of 10 milliliters, with final concentrations of 1% agarose, 5% fetal bovine serum supplemented Minimum Essential Media (Eagle), and 0.01% neutral red. Prepared samples were applied to the agarose surface in a 25 cm2 sealed flask via filter paper discs.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 15, 1990
- Accession Number
- ADB160703
Entities
People
- R. D. Hume