Production of Dengue 2 Envelope Protein in the Yeast Saccharomyces Cerevisiae. Phase 1

Abstract

The four serotypes of dengue viruses are a leading cause of morbidity throughout the tropics and subtropics. For use in diagnostics and in subunit vaccines, a reliable and inexpensive source of dengue antigens is required. We have evaluated the ability of the yeast Saccharomyces cerevisiae to express antigenic dengue envelope (E) glycoprotein. We subcloned the E gene of dengue-2 strain PR159SI into six S. cerevisiae expression vectors which encompass both constitutive and regulated promoters, with and without secretion signal peptide- encoding sequences. We have screened by Western blots both intracellular and secreted proteins of yeast transformed with the recombinant yeast-dengue genes for antigenic dengue E protein. Our results demonstrate that the yeast S. cerevisiae will express and secrete antigenic dengue E glycoprotein. Dengue 2 virus, Envelope glycoprotein, Yeast, Saccharomyces cerevisiae.

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Document Details

Document Type
Technical Report
Publication Date
Feb 15, 1990
Accession Number
ADB170320

Entities

People

  • John M. Ivy
  • Kathy Houtchens

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Amino Acids
  • Biological Factors
  • Biotechnology
  • Coding
  • Dengue
  • Fungi
  • Genetic Code
  • Genetics
  • Materials
  • Molecular Weight
  • Polymerase Chain Reaction
  • Production
  • Proteins
  • Rna Viruses
  • Secretion
  • Vaccines
  • Viruses

Fields of Study

  • Biology
  • Engineering

Readers

  • Microbial Pathology
  • Oncology (Cancer Research).
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology