A Gene Probe Amplification System Optimized for Direct Imaging Detection.
Abstract
The key feasibility elements have been completed that are expected to lead to a portable field system for rapid water analysis using gene probe amplification techniques. A filtration system has been devised to separate E. coli bacteria from other species and debris in lake water samples. Cell lysing techniques for E. coli have been investigated and compared for efficiency. Gene amplification techniques also have been investigated and optimized for detection of E. coli DNA. Separation and detection of the amplification products were conducted using gel electrophoresis and silver staining. Lysing methods developed have reduced the time required for this step severalfold and may be reduced to a negligible level in Phase II research. Based on this Phase I feasibility investigation, we estimate that a field portable system may be developed for water quality analysis that requires fewer than 2 to 3 hours.... Water, Pathogen detection, Gene probe, E. Coli, SBIR, RA II.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 15, 1992
- Accession Number
- ADB170987
Entities
People
- Larry J. Moore