Glycosphingolipids as Putative Receptors of Staphylococcal Enterotoxin B in Human Kidney Proximal Tubular Cells

Abstract

We have investigated the binding of 125 I staphylococcal enterotoxin-B (SEB) to glycosphingolipids (GSL) from cultured human kidney proximal tubular cells employing GSL overlay technique and microplate quantitative assay. Structural studies of the putative GSL receptor for SEB was performed employing high performance liquid chromatography (HPLC), gas liquid chromatography, mass spectrometry (GC-MS) of Trimethylsilyl derivatives of methylglycosides, fatty acids and sphingosines. Permethylation analysis of sugars was carried out employing GC-MS. Our results are: 1. Out of twelve GSL present in human kidney, only a diglycosyl ceramide specifically bound to SEB. 2. The chemical composition of the putative receptor: carbohydrates, glucose, galatose, sphingosines, dl8:2, d23:0, fatty acids, C16:0, C18:1. RA 1, Glycosphingolipids, Receptors, Kidney proximal tubular cells, High performance thin layer chromatography.

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Document Details

Document Type
Technical Report
Publication Date
Dec 26, 1992
Accession Number
ADB171619

Entities

People

  • Subroto Chatterjee

Organizations

  • Johns Hopkins University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Amides
  • Biomedical Research
  • Blood
  • Carbohydrates
  • Cells
  • Chemical Composition
  • Chemistry
  • Chromatography
  • Fatty Acids
  • Kidneys
  • Laboratory Animals
  • Liquid Chromatography
  • Mass Spectra
  • Mass Spectrometry
  • Materials
  • Spectrometry
  • Spectroscopy

Fields of Study

  • Chemistry

Readers

  • Analytical Chemistry
  • Microbial Pathology
  • Molecular and Cellular Biology