Glycosphingolipids as Putative Receptors for Staphylococcal Enterotoxin-B in Human Kidney Proximal Tubular Cells.
Abstract
Staphylococcal enterotoxin-B is an exotoxin capable of producing severe food poisoning in experimental animals and man. The proximal tubular (PT) cells in the kidneys of experimental animals are the major site of this toxin. We have found that 125I-SEB is specifically bound to a digalactosylceramide present in human kidney and cultured human kidney PT cells. In contrast, 125I-SEB binding to glycosphingolipids (GSL) derived from rat kidney, human brain, or human intestine was not observed. The absence of this receptor in rat kidney may suggest a possible biochemical basis of immunity to SEB in such animals. We also found that the biological responses of PT cells consistent to the binding of 125I-SEB (and certain SEB-peptide domains) are: release of lactate dehydrogenase, cell death, and the production of nitric oxide. The latter concurs with pathophysiological observations of SEB-induced shock and the dilation of the blood vessels in the kidney in monkeys. Glycosphingolipids, Receptors, Kidney proximal tubular cells, High performance thin layer chromatography.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 20, 1994
- Accession Number
- ADB190882
Entities
People
- Subroto Chatterjee
Organizations
- Johns Hopkins University