An Automated Hollow Fiber System for the Deglycerolization of Thawed Frozen Human Blood. Phase 1.
Abstract
This Phase I program demonstrated the feasibility and potential advantages of a new technical approach for the deglycerolization of thawed frozen human blood. This approach uses a hollow fiber separator, modified roller pumps, and a new blood-saline mixing device incorporated into a fully automated system. The hollow fiber separator removes glycerol and saline wash solution in an efficient recirculation process using serial dilutions followed by fluid and glycerol removals. System requirements and specifications were established; system performance was analytically modeled; the system and its key components were designed; the new hollow fiber separators, recirculation bag, and blood- saline mixer were fabricated and tested; a complete prototype manually-operated system was constructed: and this system was tested with bovine and thawed frozen human blood. The requirements for glycerol removal with acceptable levels of hemolysis and intracellular potassium were achieved. All of the Phase I tasks were successfully completed and the objectives were met. All of the requirements established by the military for the future complete deglycerolization system appear to be achievable based on the results of this Phase I program.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 13, 1994
- Accession Number
- ADB190895
Entities
People
- Thomas C Robinson