Prolonged Preservation of Human Platelets for Combat Casualty Care.
Abstract
This report describes a method for extended storage of human platelets. The platelet storage is performed at 4 deg C or with cryopreservation to eliminate the bacterial contamination problems associated with conventional storage. To abrogate platelet storage lesions which occur during the 4 deg C storage, the cells are treated with second messenger effectors which stabilize the platelets by inhibiting the biochemical events which lead to storage lesion thus, the platelets remain active. Platelets treated with this stabilization mixture display activity profiles, following 6 days of storage at 4 deg C, equivalent to fresh platelets (i.e. 100% recovery of activity). In contrast, platelets stored at 4 deg C in the absence of the stabilization mixture yield <60% recovery of cell number, approx. 10% response to agonist stimulation and approx. 10% response to hypotonic stress. Moreover, platelets stored by conventional methods for 5 days, the maximal allowable, display only between 50% -85% agonist response and approx. 60% hypotonic response. Platelets, treated with the stabilization mixture and cryopreserved via the conventional DMSO method, yield > 95% recovery of cell number, approx. 70% response to collagen stimulation and approx. 75 % response to hypotonic stress, as compared to fresh platelets. The results of these experiments demonstrate that the implementation of this method allows platelets to be stored at 4 deg C or via cryopreservation for extended periods, without loss of activity.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 15, 1994
- Accession Number
- ADB196220
Entities
People
- Jerome Connor
- Stephen Livesey