Cytokines, Neovascularization and Breast Cancer
Abstract
Breast cancers can not grow larger than several millimeters in diameter without the ingrowth of new blood vessels (angiogenesis). Recently, Interleukin 8 (IL-8) has been found to be an angiogenic factor. The general hypothesis for this project is that breast cancer cells are capable of producing angiogenic factors (AF) which support tumor growth and metastasis. In order to test our hypothesis, we are examining the expression of IL-8 antigen in human breast tissue using immunohistochemical methods, examining breast cancer cells grown in culture, and establishing an in vivo model in immunodeficient mice. In the first year of this grant, we have examined 29 breast cancers, S in situ cancers, and 11 normal breast tissues for IL-S expression. In addition, blood vessel staining was done using an antibody to vWF. The results confirmed the expression of IL-8 by breast cancer cells. Currently, we are correlating IL-8 expression with angiogenesis. Additional specimens are being processed. We have begun testing the ability of breast cancer cells in vitro to express IL-8. Nine cell lines have been tested. We have found that in breast cancer cells, IL-1 is a potent inducer of IL-8 production. These results support our hypothesis that breast cancer cells produce AF and likely result in tumor growth and metastasis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1995
- Accession Number
- ADB209449
Entities
People
- Scott H. Kurtzman
Organizations
- University of Connecticut Health Center