Feasibility Assessment of a Structurally Closable, Automatable Technique, for the Deglycerolization of Frozen RBC (Human). Phase 2

Abstract

A prototype instrument and associated sterile disposable tube sets for the deglycerolization of frozen red blood cells (RBC) were built and tested. The instrument is capable of prediluting and washing up to two units of blood per tube set. Our results show that when the performance of our machine is compared to centrifugation we obtain equivalent glycerol removal. Further intracellular Na+ and K+ of the washed RBCs is acceptable. Our RBC recovery is low (about 70% versus about 85% using centrifugation) and the supernatant haemoglobin concentration of the washed blood high (200 mg dL-1 versus about 100 mg dL-1 using centrifugation).

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1996
Accession Number
ADB210895

Entities

People

  • Franco Castino

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Assembly
  • Biomedical Research
  • Blood
  • Blood Cells
  • Blood Volume
  • Cells
  • Fluid Mechanics
  • Hemoglobin
  • Manufacturing
  • Models
  • Plastic Explosives
  • Polymers
  • Pressure Transducers
  • Prototypes
  • Steady State
  • Test And Evaluation
  • Test Methods

Readers

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  • Immunology