Identification of Protective Brucella Antigens and their Expressions in Vaccinia Virus to Prevent Disease in Animals and Humans.
Abstract
Vaccinia-Brucella recombinants expressing the Brucella GroEL, HtrA and Cu/Zn SOD proteins were used to immunize mice and the animals were tested for protection against a virulent B. abortus challenge. The Wyeth vaccinia strain expressing GroEL recombinant was unable to proliferate in mice and did not protect. The Western Reserve vaccinia strain expressing HtrA or SOD were able to replicate in mice but did not protect after 1 or 2 vaccinations. It was not possible to detect a CMI response to HtrA or SOD in these mice which may account for lack of protection. A synthetic early-late promoter is now being used to generate vaccinia constructs in order to overcome this problem. Homologs of the E. coli YajC and SecD were identified in Brucella and vaccinia recombinants are being generated nsing the corresponding genes. Genes for the Brucella 18kDa lipoprotein and a 14kDa protein have been cloned to create vaccinia recombinants with them. The Baculovirus expression system is being employed to produce large quantities of Brucella antigens for in vitro and in vivo testing. Fractionation of Brucella lysates is being used as an alternative method to find protective antigens; 4 additional antigens have been identified by this method.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1996
- Accession Number
- ADB212337
Entities
People
- Gerhardt G. Schurig