Glycosphingolipids as Putative Receptor for Staphylococcal Enterotoxin-B in Cultured Human Kidney Cells.
Abstract
Staphylococcal enterotoxin-B (SEB) is a common enterotoxin that can cause diarrhea and death in man. Since, we found that digalactosylceramide is most probably a putative receptor for SEB in cultured human kidney proximal tubular cells, it was used to develop a specific and sensitive assay for SEB (enzyme linked receptor-based immunodot) in human fluids, plasma and urine. Digalactosylceramide was immobilized on a polyvinyledene difluoride membrane and the membrane was subsequently incubated with primary and secondary alkaline phosphatase conjugated antibodies. A positive reaction was discerned as a blue spot. As little as 1 ug/ml SEB could be detected in the assay. SEB did not bind to structurally related glycosphingolipids, indicating specificity. This assay was specific for SEB, but not for SEA and TSST-I. Further work is in progress to study the application of this method in the field. Our findings will be of potential value for the food industry, and to determine toxemia in our soldiers.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1996
- Accession Number
- ADB215486
Entities
People
- Subroto Chatterjee
Organizations
- Johns Hopkins University