Expression and Regulation of the Retinoic Acid Receptor Beta Gene in Human Mammary Epithelial Cells
Abstract
We have performed BRARE (beta retinoic acid response element) gel shifts and supershifts, using antibodies for RAR-alpha and RAR BETA, with probes to nuclear extracts from 2 normal and 3 tumor lines. We have performed these analyses with RAR BETA transduced breast cancer cells, MCF7 and MDA-MB-231. We have studied the effects of retinoic acid (RA) on growth of normal human mammary epithelial cells (HMECs) in comparison to tumor cells. We have begun experiments to examine the in vivo behavior of breast cancer cell lines transduced with the RAR BETA gene through nude mice studies. By differential display, we have identified eight genes regulated by retinoic acid in either breast cancer cell lines and/or normal HMECs. Our major, current goals are to confirm the hypothesis that RAR BETA is a tumor suppressor gene for breast cancer and to understand the receptor and ligand interactions necessary for invoking and maintaining growth control and/or a differentiated phenotype.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1996
- Accession Number
- ADB218953
Entities
People
- Karen Swisshelm
Organizations
- University of Washington