Selection of Human Antibody Fragments which Bind Novel Breast Tumor Antigens
Abstract
For this work, we proposed to isolate and characterize a large assortment of high affinity human antibody fragments that bound to specific breast cancer antigens and to normal antigens that are overexpressed on cancer cells. Human antibodies isolated using phage display would be used for early sensitive diagnosis of node-negative breast cancer patients, for immunotherapy prior to growth of large tumor mass, and as adjuvant therapy for minimal residual disease. Human antibody repertoires were to be created from the mRNA of healthy individuals using the polymerase chain reaction, and cloned to create a very large and diverse phage antibody library. This phage antibody library would contain a greater number of antibodies against more epitopes on more antigens. The affinities of the initial isolates would also be higher. Antibodies that recognize antigens which are overexpressed or unique to breast carcinomas would be isolated by selection on breast tumor antigens or cell lines and characterized with respect to affinity and specificity. Affinities were to be increased by mutagenesis of the antibody genes, construction of mutant phage antibody libraries, and selection on tumor cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1996
- Accession Number
- ADB221274
Entities
People
- James D. Marks
Organizations
- University of California, San Francisco