Regulation of Estrogen Receptor Transcription in Breast Carcinoma.
Abstract
Estrogen Receptor Factor-i (ERF-1) is a transcription factor present in estrogen receptor (ER)- positive breast and endometrial carcinoma cell lines that binds to the untranslated leader sequence of ER, thus inferring a role for this protein in the transcriptional regulation of ER. There are two binding sites for ERF-1 in the untranslated leader sequence; a high affinity distal site and a lower affinity proximal site. The minimal ERF-1 binding site was mapped using double point mutants in competitive gel shift assays. The molecular weight of ERF-1 was determined using gel renaturation with competitors and UV crosslinking techniques. A protein purification strategy using ion-exchange and DNA affinity chromatography was developed to purify ERF-1 from MCF7 human breast carcinoma cells. Purified ERF-1 is currently being microsequenced and the sequence information will be used to clone the cDNA for ERF-1 from a MCF7 expression library.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1996
- Accession Number
- ADB221777
Entities
People
- Ronald J. Weigel
Organizations
- Stanford University