Fellowship To Study the Involvement of Heat Shock Proteins in Drug Resistance in Human Breast Cancer.
Abstract
Heat shock proteins (hsps) are induced in cells in response to environmental stresses. It has been shown that breast cancer cells sometimes express high levels of hsp27, which may augment the aggressiveness of these tumors and make them more resistant to treatment. This study was designed to determine the role of hsp27 in resistance to specific chemotherapeutic drugs, and to begin dissecting the regulation of hsp27 in human breast cancer cells. Toward this goal, we proposed the following specific aims: (1) Examine the regulatory mechanisms underlying the expression of hsp27 in breast cancer cell lines and human breast tumors. (2) Identify genes whose expression is associated with hsp27 effects on proliferation and drug resistance, as well as proteins interacting directly with hsp27. (3) Target positive and negative hsp27 transcriptional regulatory factors identified in Aims 1 and 2 to interfere with hsp27 expression. (Years 2-3). The work addressing Aim 1, carried out in our laboratory between 30 January 1995 to 30 January 1996 is the subject of this report. The first year of investigation was spent addressing specific aim 1. As discussed in the grant, we cloned the hsp27 promoter region and identified a broad area involved in basal transcription and a negative response region by deletion mapping.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1996
- Accession Number
- ADB222413
Entities
People
- Suzanne A. Fuqua
Organizations
- University of Texas Health Science Center at San Antonio