Novel Membrane System to Automatically Deglycerolize Thawed Frozen Human Blood
Abstract
Improved methods for frozen storage of glycerolized red blood cells (RBCs) are needed both to meet military and civilian needs in periods of high demand. Existing deglycerolization systems are labor intensive and considered 'open' systems resulting in a maximum storage of 24 hours following washing. AHT's thawed blood processing system (TBPS) has been developed through optimization of a disposable configuration and automated hardware console. AHT has met the essential element of the TBPS development plan and demonstrated significant improvement in the quality of blood product washed with the TBPS as well as completed the design, assembly and validation of an automated console to process frozen thawed blood. Key determinants of performance of the TBPS have been determined to be the choice of the flat filter media used and the type of spinning rotor used to mix the blood within the filter. In its final configuration, the ThPS typically yields a washed frozen-thawed blood product with a hematocrit of 60-70%, recovery of 80-90%, and supernatant hgb levels of l50-300mg/dL. The Phase H development has lead to a closed, sterile washing system for thawed blood and should allow for extended storage thawed REC products pending future incorporation of additive solutions.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1997
- Accession Number
- ADB223531
Entities
People
- John J. Meserko