Novel Membrane System to Automatically Deglycerolize Thawed Frozen Human Blood. Phase II

Abstract

Improved methods for frozen storage of glycerolized red blood cells (RBCs) are needed both to meet military and civilian needs in periods of high demand. Existing deg!ycerolization systems are labor intensive and considered 'open' systems resulting in a maximum storage of 24 hours following washing. The washing of blood cells with flat sheet membrane filters has been proven effective by the Advanced Haemotechnologies. AHT'S thawed blood processing system development in year one of the Phase II proposal has focused on optimizing the disposable operation and configuration as well as developing hardware. Suggested key operational parameters include the total volume concentration of blood in the filter and shear induced hemolysis of the blood during washing. Advances in each of the areas were made for washing fresh and frozen thawed red blood cells. Successful completion of the Phase H development will lead to a closed, sterile washing system for thawed blood and should allow for extended storage thawed RBC products.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1996
Accession Number
ADB223532

Entities

People

  • Abdul Balogun
  • John J. Meserko
  • Joseph Wyse
  • Patrick Cullen
  • Philip G. Ralston

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  • Air Platforms
  • Biomedical
  • Human Systems
  • Space

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  • Blood
  • Blood Cells
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  • Geotechnical Engineering.
  • Immunology