In Vivo Analysis of Alternative Pre-mRNA Splicing.
Abstract
Alternative splicing of pre-mRNA transcripts is normally an important mechanism to regulate the expression of many different protein isoforms including transcription factors and other regulatory proteins. Mis-regulation of alternative splicing can lead to the expression of inappropriate forms of such proteins in cells and has been shown to play a role in the establishing the cancerous state, enhancing the virulence of certain cancers, or inhibiting the treatments of cancer. The mechanisms that regulate alternative splicing have been investigated in Drosophila using the alternatively spliced myosin heavy chain gene as a model. The unique molecular and genetic tools provided by this system, have allowed the characterization of the elements that participate in permitting alternative splicing in general and have generated a model to explain the overall regulation of alternative splicing in this gene. The findings here are likely applicable to the understanding of other complex alternatively spliced genes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1996
- Accession Number
- ADB225042
Entities
People
- David M. Standiford
Organizations
- Fox Chase Cancer Center